Fig. 1.

e1a induces hypoacetylation of H3K18. Contact inhibited human primary IMR90 fibroblasts were mock-infected or infected with Ad5 dl1500 (e1a only). 24 hours p.i., cells were fixed and immunostained with antibodies to e1a (green), either (A) H3K9ac (red) or (B) K3K18ac (red), and 4´,6´-diamidino-2-phenylindole (DAPI) to stain nuclei. Merges of green and red signals are also shown. (C) Fold change in histone modifications after dl1500-infection of IMR90 cells determined by mass spectrometry (fig. S2). Western blots of histones from (D) IMR90 cells infected with dl312 (no e1a) or dl1500 (e1a only) and (E) HeLa cells transduced with the indicated retrovirus vectors or dl1500-infected.