Figure 3.

Small angle X-ray scattering (SAXS) analysis of CHMP3. (A) SAXS profiles of CHMP3 in ⁰HBS (dotted black line) and ⁵⁰⁰HBS (dotted red line). Log of scattered intensity is shown vs. log of s (s = 4πsin(θ)/λ, 2θ = scattering angle and λ = wavelength), log₁₀s scale is chosen for aiding the visualization of the differences between the scattering curves at high as well as low s values. SAXS profiles calculated from the the ab initio models are shown as solid black and red lines. (B) The ab initio calculated model of CHMP3 in ⁰HBS buffer conditions reveals an elongated L-shaped molecule. Two orientations rotated by 90 °are shown (left two panels). The core of the CHMP3 crystal structure (grey ribbon) composed of an elongated four helical bundle was fitted manually into the molecular envelope calculated from the SAXS model (right panel). (C) The calculated model of CHMP3 in ⁰HBS buffer conditions reveals an elongated 106 Å long molecule. Two orientations rotated by 90° are shown (left two panels). The core of the CHMP3 crystal structure (grey ribbon) was fitted into the elongated structure (right panel). The N-terminal residue is indicated in blue and the C-terminla residue in red.