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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Pharmacol Res. 2009 Jun 18;60(4):229–236. doi: 10.1016/j.phrs.2009.06.003

Figure 5. Nrf2 activates rat Gclc gene promoter activity through ARE3.

Figure 5

H4IIE cells were transfected with 0.8 µg of either of the three Gclc ARE elements or the ARE mutant, 0.06 µg of pcDNA3.1-Nrf2 (Nrf2) expression plasmid along with 0.02 µg of pHRL-CMV Renilla luciferase plasmid as a control for transfection efficiency. Nrf2 response of each reporter construct is indicated by fold change of activity versus the activity of pcDNA3.1 control plasmid that was co-transfected with it. Nrf2 over expression failed to activate the ARE3 mutant construct. Mean values of relative luciferase activity from at least three independent experiments, each carried out in duplicate, are shown with the ± SEM as detailed in “Materials and Methods”. ‘a’, p ≤0.01 relative to ARE3 alone (Tukey’s post-hoc analysis). Mean difference of the Nrf2 response for the ARE3 mutant group is significant from control group at p ≤ 0.01.