Table 3.
Sequence of Inserts in the pGL4 Minimal Promoter Vector
| Plasmid | Insert (5’→3’) |
|---|---|
| pGL-3xARE1 | GATTTACAATGACTAGACACACGTAGATTTACAATGACTAGACACACGTAGATTTACAATGACTAGACACACGTA |
| pGL-3xARE2 | TCCTGACAGTGAGTTGACACTCTGGTCCTGACAGTGAGTTGACACTCTGGTCCTGACAGTGAGTTGACACTCTGG |
| pGL-3xARE3 | GAGTCACGGTGAGGCGGCACGGCGCGAGTCACGGTGAGGCGGCACGGCGCGAGTCACGGTGAGGCGGCACGGCGC |
| pGL-3x embedded AP1 | GTTGAGTCACGGTTGAGTCACGGTTGAGTCACG |
| pGL-3xARE3m | GAGTCACGGGTCGGCGTAACGGCGCGAGTCACGGGTCGGCGTA ACGGCGCGAGTCACGGGTCGGCGTAACGGCGC |
a
The underlined letters represent those nucleotides that form the minimal functional ARE or AP1 element. The mutated ARE3 core sequence is in bold.