Figure 2.
Co-culture with 3T3-L1 adipocytes provides significant protection against drug treatment to 8093 leukemia cells. A: Number of viable leukemia cells after 72 hours of exposure to vincristine (5 nM), nilotinib (20 nM), daunorubicin (35 nM), or dexamethasone (25 nM) while in co-culture with 3T3-L1 fibroblasts (hatched bars) or adipocytes (solid bars), compared to culture alone (gray bars). B: Proliferation of 8093 cells in TransWells over no feeder (dotted gray line, triangles), fibroblasts (dashed line, open circles), and adipocytes (solid line, closed circles, p=n.s. for all comparisons; n=5 experiments performed in triplicate). C: Viable 8093 cells during exposure to 5 nM vincristine while in TransWells over adipocytes (solid line), fibroblasts (black dashed line), or no feeder layer (gray dotted line; n=5 expts done in triplicate) *p<0.05, ***p<0.005 adipocyte vs. fibroblast feeder layer, paired t-test.