Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Cancer Res. 2009 Sep 22;69(19):7569–7576. doi: 10.1158/0008-5472.CAN-09-0529

Figure 1. miR-34a inhibits the expression of multiple oncogenes and binds to their 3′UTR in brain tumor cells.

Figure 1

A) Glioma cells and stem cells, medulloblastoma cells or astrocytes were transfected with either pre-miR-34a or pre-miR-con for 72 hrs. c-Met, Notch-1, Notch-2 and CDK6 protein levels were measured by immunoblotting. The results show that miR-34a expression downregulates c-Met, Notch-1, Notch-2 and CDK6 protein levels (right panels). The predicted seed matching between miR-34a and the oncogenes' 3′UTR sequences is shown in the left panels. B) Glioma cells were transfected with pre-miR-34a or pre-miR-con for 24 hrs prior to transfection with either c-Met 3′UTR, Notch-1 3′UTR, Notch-2 3′UTR or control reporter plasmids together with β-Gal plasmids for 48 hrs and 3′UTR reporter activity was measured by a luciferase assay and normalized to β-Gal. The results show that miR-34a expression downregulates c-Met, Notch-1 and Notch-2 luciferase activities in the cells. NICD = Notch intracellular domain. * = p<0.05.