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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Clin Cancer Res. 2009 Sep 22;15(19):6096–6105. doi: 10.1158/1078-0432.CCR-09-0589

Figure 1. ABT-737 increases radiation-induced apoptosis in H460 cells.

Figure 1

(A) H460 cells were pretreated with 100 nM ABT-737 for 2 hours, followed by radiation (0, 5, or 20 Gy). Western blot analysis for active caspase-3 was performed after 24 hours. Actin immunoblot was used for normalization. (B) H460 cells were treated with ABT-737 (500nM for 2 hours) and immediately irradiated with 5 Gy or 20 Gy. After 24 h, cells were stained with Annexin V and propidium iodide, and analyzed by flow cytometry. This experiment was done in triplicate and representative diagrams of Annexin-V assays are shown. (C) Quantitative measurement of Annexin V flow cytometry analyses showing positive apoptotic cells in response to radiation/ABT-737. Columns, mean; bars, S.D. (D) H460 cells were treated with ABT-737 (500nM for 2 hours) and immediately irradiated with 5 Gy or 20 Gy. 24 hr later, they were stained with trypan blue. The total number of cells and the number of stained cells were determined using a hemocytometer under a microscope. This was repeated thrice. Columns, mean; bars, S.D.