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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Cancer Res. 2009 Aug 25;69(18):7311–7319. doi: 10.1158/0008-5472.CAN-09-1077

Figure 3. Genetic-selective inhibition of cell proliferation by siRNA targeting the PI3K/Akt pathway.

Figure 3

A) FTC133, OCUT1, SW1736 or KAT18 cells were infected with lentivirus expressing Akt-1/2 siRNA or control lentivirus packaged with empty vector. Stable cell pools, selected with puromycin, were lysed and immunoblotted with corresponding antibodies as described in Materials and Method. The antibody against β-actin was used for quality control of Western blotting. B) Similar procedure was performed using specific siRNA to knock down mTOR. C) Comparison of proliferation rates of FTC133 OCUT1, SW1736 and KAT18 cells with/without stable knock-down of Akt-1/2 or mTOR as indicated. Stable cell pools were seeded into 96-well plates and cell proliferation rate was detected with MTT assay. The OD value (relative cell number) was measured daily over a 5-day time course.