Fig. 1.
Influence of genetic background on the expression of AQP isoforms in kidneys of adult C57BL/6J and CD-1 mice. a Real-time RT-PCR quantification of AQP isoforms (AQP0 to AQP12) in C57 vs. CD-1 mouse adult kidneys (n = 5 individual kidneys for each genotype; *P < 0.05). The values are expressed in [target/reporter gene (Gapdh) ratio] × 103. b Immunostaining for AQP2 (a–d), Ser256 pAQP2 (e–f), and AQP1 (g–h) in adult kidneys from CD-1 (left panels) and C57 (right panels) mice. The representative micrographs show a higher staining intensity for AQP2 and pAQP2 in the collecting ducts (outer medulla) of the CD-1 kidneys, whereas the staining intensity for AQP1 in the proximal tubules and medullary structures (including descending vasa recta and descending thin limbs of long loop nephrons) is similar (g–h). Bars 50 μm