Table 1.

Ranking and relative mRNA expression stability of reference genes in the developing mouse kidney

Rank	geNorm		Normfinder		BestKeeper
	Gene	M value	Gene	Stability value	Gene	CV [% CP]	SD [± CP]
C57
1	Gapdh	0.593	Gapdh	0.349	Gapdh	2.38	0.55
2	Hprt1	0.614	Hprt1	0.360	Hprt1	2.81	0.50
3	Actb	0.625	Actb	0.478	CypA	3.24	0.59
4	CypA	0.652	CypA	0.563	Actb	3.34	0.70
CD-1
1	Gapdh	0.576	Gapdh	0.289	Gapdh	2.46	0.44
2	Hprt1	0.580	Hprt1	0.297	Hprt1	2.65	0.59
3	CypA	0.618	CypA	0.318	Actb	2.90	0.51
4	Actb	0.629	Actb	0.336	CypA	2.92	0.54

Genes are ranked based on their M value, stability value, and CV and SD calculated by geNorm, Normfinder, or BestKeeper algorithms, respectively. A lower value indicates more stable reference gene expression. Gapdh glyceraldehyde-3-phosphate dehydrogenase, Hprt1 hypoxantine guanine phosphoribosyl transferase 1, CypA cyclophilin A, Actb β-actin

Five cDNA samples were amplified for each stage of development.