Table 2.
Influence of the strain on the relative mRNA abundance of aquaporin isoforms in adult mouse kidney
| Gene | PS1 | PS2 | ||
|---|---|---|---|---|
| C57 | CD-1 | C57 | CD-1 | |
| Aqp0 | ND | ND | ND | ND |
| Aqp1 | 62.6 ± 5.5 | 90.3 ± 8.9* | 82.5 ± 9.9 | 89.3 ± 6.5 |
| Aqp2 | 13.1 ± 1.7 | 24.9 ± 2.3* | 20.5 ± 3.3 | 33.0 ± 2.2* |
| Aqp3 | 24.0 ± 1.8 | 35.4 ± 2.8* | 24.0 ± 1.9 | 46.4 ± 7.0* |
| Aqp4 | 2.3 ± 0.3 | 5.7 ± 0.5* | 2.3 ± 0.3 | 3.7 ± 0.6* |
| Aqp5 | ND | ND | ND | ND |
| Aqp6 | 4.2 ± 0.4 | 7.3 ± 0.9* | 4.8 ± 0.6 | 3.8 ± 0.3 |
| Aqp7 | 6.7 ± 0.6 | 11.0 ± 1.3* | 4.5 ± 0.3 | 7.6 ± 0.3* |
| Aqp8 | 0.2 ± 0.0 | 0.3 ± 0.1 | 0.3 ± 0.0 | 0.6 ± 0.1 |
| Aqp9 | ND | ND | ND | ND |
| Aqp10 | ND | ND | ND | ND |
| Aqp11 | 24.9 ± 1.8 | 26.8 ± 3.0 | 28.4 ± 2.6 | 37.0 ± 3.3 |
| Aqp12 | ND | ND | ND | ND |
The RNA expression levels were compared after adjustment to glyceraldehyde-3-phosphate dehydrogenase (Gapdh; ×103) as reporter gene. Significant differences (*P < 0.05) between C57 and CD-1 are indicated for both sets of primers
PS1 and PS2 primer set 1 and 2, ND non-detected (threshold value fixed <0.05)