Fig. 5.

Analysis of ex vivo-isolated LSEC after culture. LSEC were enriched by magnetic cell sorting using ME-9F1. a After in vitro culture over night morphology was assessed by phase contrast microscopy (bar = 50 μm). The uptake of AcLDL by LSEC was analyzed by b fluorescence microscopy (AcLDL, green; nucleus staining with Hoechst 33342, blue) or c flow cytometry after counterstaining with ME-9F1. d The expression of CD31, VE-cadherin and Meca32 antigen (bold line) was analyzed by flow cytometry at day 0 and 6 after in vitro culture. The respective isotypes were stained for negative control (thin line). Data shown are representative from least four independent experiments