Fig. 6.

Endothelial growth and transendothelial migration of T cells after ME-9F1 binding to the endothelium. a sEND were treated with 50 μg/ml ME-9F1 or control antibody during culture. Phase contrast pictures taken after 24 and 48 h are representative for two independent experiments (bar = 50 μm). b bEND5 grown to confluence on a transwell membrane were treated with ME-9F1 alone or with subsequent cross-linking with anti-rat antibody prior to the transendothelial migration assay. T cells were added to the upper chamber of the transwell and transmigrating T cells harvested and quantified after 90 min. Data show one representative experiment out of two independent experiments with duplicate determination