Fig. 1.

Physcomitrella patens SBP-box genes PpSBP1 and PpSBP4 and their disruption. a Phylogenetic relationship of PpSBP1 and PpSBP4 and other P. patens SBP-box genes as based on the conserved SBP-domain. C. reinhardtii CRR1 functioned as outgroup. The LG1-subfamily is boxed in grey. Only bootstrap values over 50% are shown. b Absence of transcript validation by RT-PCR of the ppsbp1-1 and ppsbp4-1, -2 and -3 disruptant lines. Presence of the respective transcripts in wild type (wt) is shown for comparison and amplification of PpRAN transcript as reference for quantification