Figure 1.

Evaluation of cell death mode by AO and EB staining. U937 cells were cultured for 48 h with different concentrations of Ara-C. The cells were stained with EB and AO and observed using fluorescence microscopy. (a) Living cells have normal-shaped nuclei with green chromatin. (b) Early apoptotic cells have shrunken nuclei with condensed green chromatin. (c) Late apoptotic cells have condensed and fragmented nuclei containing orange chromatin. (d) Necrotic cells have normal-shaped nuclei containing orange chromatin.