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. 2009 Jun 22;587(Pt 16):4105–4119. doi: 10.1113/jphysiol.2009.175554

Figure 1. Angiomotin expression in rat skeletal muscle.

Figure 1

A, immunofluorescence staining on plantaris muscle cryosections revealed that angiomotin protein is expressed in both capillaries (stained for isolectin-B4) and skeletal myofibres (unstained) as respectively illustrated by the arrowheads and arrows. Scale bar, 50 μm. B, PECAM/CD31 protein was detected in skeletal muscle by Western blotting. Probing of the membrane without any primary antibody ensured the specificity of PECAM detection. C, Western blot illustrating angiomotin and PECAM protein expression in extensor digitorum longus, plantaris and soleus muscles. β-Actin protein detection was used as a loading control. D, densitometric analysis of PECAM expression. Data are presented as means ±s.e.m. (n= 5/muscle type). Significantly different from soleus muscle: *P≤ 0.05; **P≤ 0.01; ***P≤ 0.001. E, linear regression between angiomotin and PECAM protein expression in all muscles analysed (SOL, EDL, PLA). Abreviations: Amot, angiomotin; PECAM, platelet endothelial cell adhesion molecule-1; EDL, extensor digitorum longus; PLA, plantaris; SOL, soleus.