Plasma LH (A) and PRL (B) were measured in OVX animals in response to icv injection of E2 (open circle, n=5), G1 (filled circle, n=5) or the vehicle (triangle, n=10). In (B), PRL results are expressed after subtracting the basal value for each animal. Plasma PRL and LH were measured by ELISA and RIA, respectively.
In A: Two way ANOVA, main effect F(df) = 4.06 (2,85), P<0.05.
In B: Two way ANOVA, main effect F(df) = 5.14 (2,90), P< 0.05
*P<0.05 vs. vehicle