OVX female rats subjected to control (shcontrol, open circle n=6) or specific GPR30 (shGPR30, filled circle, n=6) RNA interference in the mediobasal hypothalamus were injected iv with 10 μg of E2. Serial blood sample collection started 5 min before drug injection (T=0). Plasma LH (A) and PRL (B) were measured by RIA and ELISA, respectively.
In A: Two way ANOVA, no main effect of virus.
In B: Two way ANOVA, main effect of virus, F(df)= 5.33 (1,45), P<0.05
* P<0.05 versus shcontrol