Figure 5.

In WT mice, all interventions led to a significant activation of the signaling molecule, Akt, whereas no change from baseline was evident in CREB^IR mice. *Among the WT mice, those who exercised (p < .0001, n = 27), received reboxetine (p = .027, n = 7), and received the combination of the two treatments (p < .0001, n = 7) expressed significantly more P-Akt immunoreactivity than those that were sedentary (p < .0001, n = 12). #WT mice also expressed significantly more P-Akt immunoreactivity than CREB^IR mice for each intervention, except for sedentary mice, who expressed the same amount between genotypes. Among the CREB^IR mice, there were no significant differences among treatments in P-Akt immunoreactivity. Because of the very small sample volumes, each mouse hippocampus was randomly assigned to a particular gel/Western film, thereby controlling for film-to-film variability. Therefore, the row of P-Akt bands and their respective Akt and GAPDH bands are a composite from several different Western blotting films. However, each P-Akt band and its respective Akt band and GAPDH band all represent the same mouse hippocampal sample (same gel lane).