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Effect of different ZnSO4 concentration on MMP-2 activity measured in the OmniMMP® fluorogenic substrate kinetic assay. ZnSO4 was incubated with 0.2 nM 64 kDa MMP-2 and 15 µM OmniMMP fluorogenic substrate at 37 °C in 50 mM Tris, pH 7.6, containing 10 mM CaCl2. Data represent means ± SD of triplicate determinations. *Statistically significant difference compared to the 10 µM ZnSO4 group, P < 0.05, one-way ANOVA with Newman–Keuls post hoc test.
