Fig. 2.
Concentration-dependent effect of PARP inhibitors (A) and MMP inhibitors (B) on MMP-2 activity using the OmniMMP® fluorogenic substrate kinetic assay. PARP and MMP inhibitors were incubated with 0.2 nM 64 kDa MMP-2 and 15 µM OmniMMP fluorogenic substrate at 37 °C in 50 mM Tris buffer, pH 7.6, containing 10 mM CaCl2 and 10 µM ZnSO4. Data represent means ± SD of three to five replicate determinations and were fitted to sigmoidal (variable slope) curves.