Table 1. Nucleotide sequences of human specific primers for real-time RT–PCR.
Gene | Primers | Sequence | Product size (bp) |
---|---|---|---|
IκBα |
Forward |
5′-AAGTGATCCGCCAGGTGAAG-3′ |
86 |
|
Reverse |
5′-GCAATTTCTGGCTGGTTGGT-3 |
|
NF-κBp65 |
Forward |
5′-TCTCCCTGGTCACCAAGGAC-3′ |
64 |
|
Reverse |
5′-TCATAGAAGCCATCCCGGC-3′ |
|
MMP-2 |
Forward |
5′-CGAATCCATGATGGAGAGGC-3′ |
87 |
|
Reverse |
5′-TCCGTCCTTACCGTCAAAGG-3 |
|
GAPDH |
Forward |
5′-CACCAACTGCTTAGCACCCC-3′ |
81 |
|
Reverse |
5′-TCTTCTGGGTGGCAGTGATG-3′ |
|
TIMP-2 |
Forward |
5′-TTCATTCGTCTCCCGTCTTT-3′ |
113 |
|
Reverse |
5′-ACCAACGTGTGTGGATCAAA-3′ |
|
MT1-MMP |
Forward |
5′-TCGGCCCAAAGCAGCAGCTC-3 |
180 |
Reverse | 5′-CTTCATGGTGTCTGCATCAGC-3′ |
The oligonucleotide sequences of real-time RT-PCR primers for Human IκBα, NF-κBp65, MMP-2, GAPDH, TIMP-2, and MT1-MMP are shown in the table.