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. 2009 Sep 26;15:1977–1987.

Table 1. Nucleotide sequences of human specific primers for real-time RT–PCR.

Gene Primers Sequence Product size (bp)
IκBα
Forward
5′-AAGTGATCCGCCAGGTGAAG-3′
86
 
Reverse
5′-GCAATTTCTGGCTGGTTGGT-3

NF-κBp65
Forward
5′-TCTCCCTGGTCACCAAGGAC-3′
64
 
Reverse
5′-TCATAGAAGCCATCCCGGC-3′

MMP-2
Forward
5′-CGAATCCATGATGGAGAGGC-3′
87
 
Reverse
5′-TCCGTCCTTACCGTCAAAGG-3

GAPDH
Forward
5′-CACCAACTGCTTAGCACCCC-3′
81
 
Reverse
5′-TCTTCTGGGTGGCAGTGATG-3′

TIMP-2
Forward
5′-TTCATTCGTCTCCCGTCTTT-3′
113
 
Reverse
5′-ACCAACGTGTGTGGATCAAA-3′

MT1-MMP
Forward
5′-TCGGCCCAAAGCAGCAGCTC-3
180
  Reverse 5′-CTTCATGGTGTCTGCATCAGC-3′

The oligonucleotide sequences of real-time RT-PCR primers for Human IκBα, NF-κBp65, MMP-2, GAPDH, TIMP-2, and MT1-MMP are shown in the table.