Figure 4.

Corneal fibroblast migration induced by VEGF-A and VEGF-C as demonstrated by a wound healing assay: Upper panel: A shows a photomicrograph of freshly created scar which is indicated as a space betweenthe two arrows, which serves as a reference for observing cell migration. B, C, D, and E are photomicrographs after 24h incubation while F, G, H, and I are photomicrographs taken after 72 h of incubation. B and F are untreated samples which serve as controls. C and G were treated with VEGF-A, while D and H were treated with VEGF-C. E and I were plates exposed to Avastin. The number of cells migrating into the scar regions were counted in all samples at time 0, 9h, 18h, 24h, 48h, and 72h of incubation and graph J was plotted.