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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2009 Jan 31;20(6):1068–1077. doi: 10.1016/j.jasms.2009.01.017

Figure 1.

Figure 1

Morphology of C. borealis brain and schematics for MALDI imaging. (a) Optical picture of isolated supraesophageal ganglion (brain) under microscope, with the major nerves and neuropils labeled. (b) Representation of the ventral surface of the isolated C. borealis brain with labeled neuropil regions showing distinctions between the fused ganglia. The brain was arranged the same way as it was observed during dissection. The most anterior portion of the brain, the protocerebrum, is a distributed structure with the protocerebral tract (PT) linking the lateral protocerebrum (not shown) with median protocerebrum (MPC), which contains two paired neuropils, anterior (AMPN) and posterior medial protocerebral neuropils (PMPN). The tritocerebrum (TC) is the most posterior region containing antenna I neuropil (AnN). Located between these fused ganglia is the deutocerebrum (DC), which includes olfactory lobe (ON) and lateral II antenna neuropil (LAN). (c) The schematic diagram of 3D imaging experiments showing serial sectioning along z-axis (numbered according to the sequence of sectioning) of the brain. (d) Comparison of dry matrix spraying and regular matrix spraying for lipid and neuropeptide detection.