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. Author manuscript; available in PMC: 2010 Jun 1.

Published in final edited form as: Genes Chromosomes Cancer. 2009 Jun;48(6):502–509. doi: 10.1002/gcc.20659

RNA interference demonstrates function of TMSB15B in cell migration to EGF. (A) PC-3MLN4 cells were treated with 3 individual control siRNAs, 2 TMSB15A siRNAs (#9 and #10) or 3 TMSB15B siRNAs (#2, #3 and #4). RT-PCR analysis using primers to TMSB15A (top panel), TMSB15B (center panel), or B2M housekeeping gene (lower panel) demonstrated isoform specificity of siRNA duplexes. (B) siRNA inhibition of TMSB15B significantly reduced PC-3 cell migration to EGF in the modified Boyden chamber assay. Data shown as mean migrated cells/low power field with error bars indicating standard error of the mean. Empty bar; untransfected cells, Horizontal striped bars; control siRNAs (thin stripe control siRNA#1, thick stripe control siRNA#3), Solid bars; TMSB15A siRNAs (solid black; TMSB15A siRNA#9, solid grey; TMSB15A siRNA#9), Striped bars; TMSB15B siRNAs (thin upward stripe; TMSB15B siRNA#2, thick downward stripe; TMSB15B siRNA#3, thick upward stripe; TMSB15B siRNA#4). * P < 0.01. (C) Parallel transfections were performed in PC-3 cells for RNA collection. RT-PCR showed decreased TMSB15B expression in all TMSB15B siRNA-treated cells. PC-3MLN4 was used as a positive control for PCR reactions (+). B2M; beta-2-microglobulin.

RNA interference demonstrates function of TMSB15B in cell migration to EGF. (A) PC-3MLN4 cells were treated with 3 individual control siRNAs, 2 TMSB15A siRNAs (#9 and #10) or 3 TMSB15B siRNAs (#2, #3 and #4). RT-PCR analysis using primers to TMSB15A (top panel), TMSB15B (center panel), or B2M housekeeping gene (lower panel) demonstrated isoform specificity of siRNA duplexes. (B) siRNA inhibition of TMSB15B significantly reduced PC-3 cell migration to EGF in the modified Boyden chamber assay. Data shown as mean migrated cells/low power field with error bars indicating standard error of the mean. Empty bar; untransfected cells, Horizontal striped bars; control siRNAs (thin stripe control siRNA#1, thick stripe control siRNA#3), Solid bars; TMSB15A siRNAs (solid black; TMSB15A siRNA#9, solid grey; TMSB15A siRNA#9), Striped bars; TMSB15B siRNAs (thin upward stripe; TMSB15B siRNA#2, thick downward stripe; TMSB15B siRNA#3, thick upward stripe; TMSB15B siRNA#4). * P < 0.01. (C) Parallel transfections were performed in PC-3 cells for RNA collection. RT-PCR showed decreased TMSB15B expression in all TMSB15B siRNA-treated cells. PC-3MLN4 was used as a positive control for PCR reactions (+). B2M; beta-2-microglobulin.