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. 2009 Oct 12;4(10):e7421. doi: 10.1371/journal.pone.0007421

Figure 1. CYP2J2 activates PPAR responses in vitro. CYP2J2 synergises with PPARα (A), PPARβ/δ or PPARγ (B) to induce PPAR reporter gene activation.

Figure 1

Dominant-negative (DN-)PPARα co-transfected into cells with CYP2J2 and PPARα abolished the ability of CYP2J2 to activate PPARα (A). HEK293 cells were transfected with PPAR reporter genes (pACO.gLuc for PPARα and –γ, and pDR-1 for PPARδ), and pcDNA-CYP2J2, pCMX-PPARα, pCMX-PPARδ, or pCMX-PPARγ alone, or co-transfected with CYP2J2 and the individual PPAR (2J2+α, 2J2+δ, and 2J2+γ). All PPAR reporter gene activation studies are represented as fold luciferase from PPAR response element transfection alone (control), normalised to total protein at 20 h post-transfection. Total plasmid DNA for transfections was normalised using pcDNA3.1 throughout. Data represents n = 9−12 replications from 4 separate experiments. * denotes p<0.05 by one-sample t-test between control and transfected cells. Inset (A) is Western blot for CYP2J2 and RT-PCR for PPARα in cells with either mock transfected (−; pcDNA3.1) or cells transfected with pcDNA-CYP2J2 and pcDNA-mPPARα (+).