Figure 4. CYP products activate PPARα. (A) 8,9-EET, and 11,12-EET, but not 14,15-EET, or 5,6-DiHETE, induce PPAR reporter gene activation in cells transfected with PPARα (with out PPARα, closed squares; +PPARα closed circles).

HEK293 cells were transfected with PPAR reporter gene in the presence or absence of PPARα. 20 h post transfection cells were treated with CYP2J2 products (0–10 µM), for a further 20 h. * denotes p<0.05 by one-way ANOVA followed by Bonferroni's post test. (B) 8,9-EET and 11,12-EET activation of PPARα responses were inhibited by co-transfection with dominant-negative DN-PPARα. Cells were transfected with a PPAR reporter alone or with PPARα, in the presence or absence of DN-PPARα. Cells were treated with CYP2J2 products as indicated (all at 1 µM). Data represents n = 9−12 replications from 3–4 separate experiments. * denotes p<0.05 by one-sample t-test between control and transfected cells.