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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Biochem Pharmacol. 2009 May 23;78(6):633–641. doi: 10.1016/j.bcp.2009.05.017

Figure 4.

Figure 4

Dnmt1 inhibition by DM72P18 ODN. Reactions containing 60 nM Dnmt1, 1.3 μM DM7 substrate, and the indicated concentration of DM72P18 inhibitor were incubated at 37 °C. At the specified times, duplicate 1 μl aliquots were removed from the pre-incubation mixture and added to an assay mixture containing 0.71 μM [methyl-3H]AdoMet to detect the remaining enzyme activity as described in Materials and Methods. (A) The percent activity remaining was plotted versus the pre-incubation time for each concentration of inhibitor. (B) The calculated t1/2 was plotted versus 1/concentration of ODN inhibitor. kinactivation=ln2/slope and kI=-1/x-intercept.