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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Biochem Pharmacol. 2009 May 23;78(6):633–641. doi: 10.1016/j.bcp.2009.05.017

Figure 6.

Figure 6

Analysis of M.HhaI and Dnmt1 binding to ODNs containing 2P by SDS-PAGE. (A) M.HhaI:2P-ODN complexes were formed in reactions containing 2.5 nM M.HhaI, 50 nM 32P-labeled 2P-ODN, and cofactor (100-200 μM). After a 30 min incubation at 37 °C, reaction mixtures were held at the indicated temperatures in SDS loading buffer (final concentration of 1% SDS, 1% BME) for 5 min prior to electrophoresis. (B) Dnmt1-ODN (DM72P18) complexes were formed in reactions containing 0.4 μM Dnmt1, 1 μM 32P-labeled DM72P18, and cofactor (100-200 μM)) and treated as described above. See text for details. Note that samples in Panel B 1-6 were electrophoresed on a separate gel from samples 7-15. Arrows indicating open (O) and closed (C) complexes in each gel are placed on the closest side of the gel.