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. Author manuscript; available in PMC: 2009 Dec 12.
Published in final edited form as: J Mol Biol. 2008 Sep 26;384(2):540–555. doi: 10.1016/j.jmb.2008.09.045

Figure 2.

Figure 2

Equilibrium Gdn-HCl titrations measured by the ellipticity in 20 mM HEPES, pH 7.2, at 20 °C. Representative data at 230 nm are shown with fits to a two-state model for the metal-free apo-AS-SOD (open squares) and apo-mAS-SOD (open circles) and with fits to a three-state model for stoichiometric Zn-AS-SOD (filled squares) and Zn-mAS-SOD (filled circles). Protein and zinc concentrations were 10 μM for AS-SOD and 5 μM for mAS-SOD.