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. Author manuscript; available in PMC: 2009 Dec 12.
Published in final edited form as: J Mol Biol. 2008 Sep 26;384(2):540–555. doi: 10.1016/j.jmb.2008.09.045

Figure 4.

Figure 4

Visible absorbance spectra of AS-SOD in 20mM HEPES, pH 7.2, at room temperature. Traces shown are protein with (A) no metal added, (B) after addition of 1 cobalt ion per monomer, (C) after pre-incubation of protein with 1 zinc ion per monomer followed by the addition of 1 cobalt ion per monomer, and (D) after pre-incubation with 6 zinc ions per monomer followed by the addition of 1 cobalt ion per monomer. The protein concentration for all four traces was 123 μM.