Figure 1.

CPMs in Mrgprd knock-in and WT mice bind IB4 but do not express either TRPV1 or CGRP. A–C, Sample immunohistochemistry of recorded CPM cells in Mrgpr ^+/+ (A), Mrgprd ^−/− (EGFPf) (B), and Mrgprd ^−/− (Cre) (C), as indicated by biotin labeling (left panels, green). Labeling of TRPV1, CGRP, and IB4 are shown for Mrgprd–IRES–EGFPf ^+/+, Mrgprd ^−/− (EGFPf), and Mrgprd ^−/− (Cre), respectively (middle panels, blue). GFP labeling is shown in the right panels (red). Arrows indicate recorded cell. Scale bar, 40 μm.