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. Author manuscript; available in PMC: 2010 Sep 18.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jun 27;387(2):245–250. doi: 10.1016/j.bbrc.2009.06.123

Fig. 3. HNE metabolism and cytotoxicity in 293T cells.

Fig. 3.

AKR1B10 delivery into 293T cells and HNE treatment were performed as described in the Materials and Methods. A) Metabolic kinetics. Cells applied with 1 or 5 µM of HNE were collected at indicated time points. HNE and DHN were extracted and quantitated by HPLC as described in the Materials and Methods. Data represent mean ± SD from three independent experiments. *, p < 0.05 and **, p < 0.01, compared to the vector control. B) HNE cytotoxicity, detected by MTT cell proliferation kit as described in the Materials and Methods. Values indicate mean ± SD from three independent experiments. * p < 0.05, compared to EGFP-AKR1B10 cells. HNE, 4-hydroxynonenal; DHN, 1, 4-dihydroxynonene; and EGFP-AKR1B10, enhanced green fluorescent protein/aldo-keto reductase family member B10 fusion protein.