Table 1. Kinetic parameters of AKR1B10 to carbonyls and glutathione conjugates.
AKR1B10 activity was measured as described in Materials and Methods. Constants were calculated by a Lineweaver-Burk plot of the reduction rate at various substrate concentrations. Vmax is expressed at nmol/mg protein/min. GS, glutathione; ND, not detectable; Cn, carbon chain length.
| AKR1B10 | |||||
|---|---|---|---|---|---|
| Substrates | Cn |
Vmax (nmol/mg/min) |
Km (µM) |
kcat (min−1) |
kcat/Km (min−1 µM−1) |
| Acrolein a | 3 | 3,122.0 ± 64.7 | 110.1 ± 12.2 | 115.9 ± 7.8 | 1.07 |
| Crotonaldehyde a | 4 | 2,647.5 ± 132.2 | 86.7 ± 14.3 | 103.4 ± 3.5 | 1.20 |
| Trans-2-hexanal | 6 | 2,658.1 ± 222.8 | 60.6 ± 18.5 | 97.4 ± 8.0 | 1.62 |
| Trans-2, 4-hexadienal | 6 | 2,160.8 ± 310.6 | 95.7 ± 5.6 | 82.8 ± 7.4 | 0.87 |
| 4-Hydroxynonenal | 9 | 3,298.7 ± 245.9 | 30.9 ± 7.1 | 120.7 ± 6.5 | 3.92 |
| GS-acrolein | 64.36 ± 5.6 | 532.8 ± 70.6 | 2.8 ± 0.1 | 0.005 | |
| GS-crotonaldehyde | 1,900.7 ± 90.9 | 245.7 ± 21.2 | 70.2 ± 3.5 | 0.29 | |
| GS-trans-2-hexanal | 2,049.0 ± 116.3 | 145.7 ± 20.5 | 71.3 ± 2.6 | 0.51 | |
| GS-trans-2, 4-hexadienal | 4,004.1 ± 362.5 | 77.6 ± 19.2 | 147.1 ± 10.1 | 1.91 | |
| GS-4-hydroxynonenal | ND | ND | ND | ND | |
a
Reference [13]