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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Biomaterials. 2009 Jul 17;30(29):5283–5291. doi: 10.1016/j.biomaterials.2009.06.032

Figure 2.

Figure 2

Gel permeation chromatography of PEG8-VS and PEG8-amine pre-reacted to dPCS ≅ 150 nm. The reaction was halted by capping remaining vinyl sulfone groups with 2-mercaptoethanol. The absolute molecular weight was determined by detection of light scattering and refractive index. A) Molecular weight (black) and refractive index (gray) of eluted polymers. The peak at 27.6 min was confirmed to be monomeric PEG8-VS in a separate run. B) Intensity of right angle light scattering (black) plotted against hydrodynamic radius. This should correspond well to the signal recorded by DLS of the pre-reacted samples. Refractive index (gray) is shown for comparison.