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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Biomaterials. 2009 Jul 17;30(29):5283–5291. doi: 10.1016/j.biomaterials.2009.06.032

Figure 4.

Figure 4

A) Cloud points of 2% (w/v) PEG8-VS, PEG8-amine (PEG8-Am) and PEG8-acrylate (PEG8-Ac) in PBS + sodium sulfate at pH 7.4. Data represent n = 3 and standard deviations were ≤ 1°C for all points. B) DLS of PEG solutions above their cloud points demonstrated that mean effective droplet diameters (dPCS values) increased linearly with time during early stages of coarsening, as expected for the early stage of phase separation. Later, an apparent plateau in coarsening was observed, possibly corresponding to a percolation-to-cluster transition. Mixing PEG8-VS with PEG8-Am just prior to phase separation had little effect on the early stage of coarsening. Pre-reaction of these components to dPCS ≅ 100 nm delayed phase separation and led to a plateau at a much lower mean diameter. Data represent three independent samples averaged over 10 runs.