Representative phase-contrast photomicrographs demonstrating swelling of PEG8-VS/PEG8-amine microspheres following buffer exchange into PBS. All microspheres were formed from pre-reacted solutions (dPCS ≅ 100 nm) diluted to 2% (w/v) in PBS + 0.6 M sodium sulfate and incubated at 37°C for: (i) 15 min at pH 6.5 without buffer exchange, (ii) 15 min at pH 6.5 with buffer exchange, (iii) 15 min at pH 7.4 without buffer exchange, (iv) 15 min at pH 7.4 with buffer exchange, (v) 105 min at pH 6.5 without buffer exchange, (vi) 105 min at pH 6.5 with buffer exchange, (vii) 75 min at pH 7.4 without buffer exchange, and (viii) 75 min at pH 7.4 with buffer exchange. Scale bars represent 25 µm.