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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Immunol. 2009 May 1;182(9):5770–5777. doi: 10.4049/jimmunol.0900127

FIGURE 2.

FIGURE 2

RETF-4NA hydrolyzing activity in chymase-spiked serum. Human serum was spiked with recombinant human chymase over the range of concentrations of enzyme indicated by the x-axis. There is a strong linear correlation between concentrations of chymase in spiked serum and observed rates of substrate hydrolysis, as reflected by change in milli-Absorbance (ΔmA410 nm) per minute. Standard curves based on this relationship allow determination of levels of chymase-like activity in native serum, as in Fig. 4.