Fig. 3. HNK modulates c-FLIP levels (A and B) and the levels of other apoptosis-related proteins (C).
The given cell lines were treated with 25 μM (H157) or 30 μM (H226 and A549) HNK for the indicated times (A) or with the indicated concentrations of HNK for 6 h (B) or for 3 h and 12 h as indicated (C). After the treatments, the cell lines were subjected to preparation of whole-cell protein lysates and subsequent Western blot analysis for detection of the indicated proteins.