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. Author manuscript; available in PMC: 2009 Oct 5.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):4624–4632. doi: 10.4049/jimmunol.0801543

FIGURE 1.

FIGURE 1

Oral administration of calcitriol to B10.RIII mice prevents retinal autoimmune disease and decreases the uveitic response. Mice challenged for EAU were gavaged with 0.5 μg/kg calcitriol or vehicle (Miglyol 812) by the prevention or the reversal regimen. A, EAU scores by funduscopic examination on day 13. B, Final EAU readout by histology on day 21. The data reflect two combined experiments with five mice per group. Score for each mouse is an average of both eyes. C, Ag-specific DTH response in mice challenged in the ear pinna with IRBP 48 h earlier. The specific response is the difference between IRBP- and PBS-challenged ears. D, Representative histopathology of EAU in the different treatment groups. Mice treated with calcitriol from day −6 have a histological picture indistinguishable from normal. E, Ag-specific cytokine production by LN cells on day 21 after immunization measured by Multiplex ELISA in 48-h supernatants. Data are shown after background subtraction of samples without Ag stimulation. Pooled supernatants were used for the assay; therefore, although data represent a group average of five mice, no error bars could be generated. Shown is a representative experiment of two with similar results. *, p < 0.01.