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. Author manuscript; available in PMC: 2009 Oct 5.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):4624–4632. doi: 10.4049/jimmunol.0801543

FIGURE 2.

FIGURE 2

Development of EAU induced with IRBP/CFA is dependent on IL-17. Mice were immunized with IRBP in CFA. A, Ag-specific cytokine secretion from LN cells as measured by Multiplex ELISA after 48-h culture. B, Cells isolated from uveitic eyes 17 days after immunization were incubated with PMA + ionomycin, and intracellular cytokine production was assayed by flow cytometry. Data shown are cells gated on CD4+ T cells. C and D, Mice were treated with neutralizing Abs to IL-17 (C) or to IFN-γ (D), or with the respective isotype. E, IFN-γ KO female mice were gavaged with calcitriol or vehicle from day −6 and immunized for EAU on day 0. Shown are scores by histology in eyes collected 17 days after immunization. Data represent one of two independent experiments with similar results. **, p < 0.01.