(a) Representative tracings of the basal dendritic tree of pyramidal neurons from the CA1 region of the HPC of Df(16)A+/−;Thy1-GFP+/− and WT Thy1-GFP+/− mice.
(b) Reduction in the total number of branchpoints of basal dendrites in Df(16)A+/−;Thy1-GFP+/− CA1 hippocampal neurons relative to WT Thy1-GFP+/− CA1 neurons.
(c) Sholl analysis of dendritic complexity using 10 µm concentric circles around the soma. A repeated measures ANOVA examining the interaction between branchpoints and distance from the soma shows an overall genotype effect indicating a significant reduction in branching prevalent throughout the dendritic tree (n = 18, P < 0.008).
(d) Representative tracings of the basal dendritic tree of pyramidal neurons from the CA1 region of the HPC of Zdhhc8+/+;Thy1-GFP+/–, Zdhhc8+/–;Thy1-GFP+/− and Zdhhc8−/− ;Thy1-GFP+/− mice.
(e) Reduction in the total number of branchpoints of basal dendrites in Zdhhc8+/−;Thy1-GFP+/− and Zdhhc8−/−;Thy1-GFP+/− CA1 hippocampal neurons.
(f) Sholl analysis of dendritic complexity. Analysis of the interaction between branchpoints and distance from the soma shows an overall genotype effect (n = 36, P < 0.01). Data are shown as mean ± S.E.M. * P < 0.05, ** P < 0.001, *** P < 0.0001.