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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2009 Aug 6;29(10):1602–1607. doi: 10.1161/ATVBAHA.109.187393

Figure 5. Platelet-FXI interactions in the presence of Zn2+, high molecular weight kininogen or β2 glycoprotein I.

Figure 5

Washed human platelets (2 × 107/ml) were incubated over surfaces of FXI or FXIa in the absence or presence of (A) Zn2+ (25µM) and high molecular weight kininogen (HK, 42 nM). Selected experiments were performed in the presence of PPACK (40 µM) or RAP (40 g/ml). P < 0.05 compared to adhesion on each respective surface in the presence of vehicle (*) or Zn2+ and HK (**). P < 0.05 compared to adhesion on FXIa with RAP in the presence of vehicle (#) or Zn2+ and HK (##). (B) Additional adhesion experiments were performed over FXI, FXIa or activated protein C (APC) in the presence or absence of plasma-derived beta2-glycoprotein I (β2GPI, 350 nM) or dimeric β2GPI (350 nM). Adherent platelets were calculated for each treatment and are reported as adherent cells/mm2 × 10−2. Adhesion numbers are reported as mean ± SEM of at least three experiments. *P < 0.05 compared to adhesion on each respective surface in the presence of vehicle.