Figure 1.
Solid-state 2H NMR spectra as a function of temperature demonstrate phase transitions of pure lipids and lipids in binary mixtures: (a) PSM-d31; (b) POPC-d31; (c) 1:1 PSM-d31/POPC; and (d) 1:1 EYSM/POPC-d31. Macroscopically oriented samples (θ = 0°) were hydrated with 10:90 (v/v) 2H2O/1H2O to a final water/lipid molar ratio of 25, as detailed in Methods. Note the sharp phase transition in (a) at 38 °C and the emergence of an additional spectral component in (c).