Chain-extension profiles (cumulative segmental projections) for POPC-d31/cholesterol binary mixtures show the influence of cholesterol on the orientational order of POPC-d31 acyl groups. The indexing (nC – i + 2) begins with the terminal methyl group (i = nC) and ends at the C2 carbon (i = 2). Results are shown for pure POPC-d31 (▲), POPC-d31 with XC = 0.2 (●), and POPC-d31 with XC = 0.33 (■) at temperatures of (a) 20, (b) 30, (c) 45, and (d) 60 °C. For POPC-d31, the chain length markedly depends on the cholesterol concentration.