Figure 4. Inhibition of GATA-3 by Syk inhibitors.
CD4+ T cells from qCII24 transgenic mice were cultured with 100 μg/ml of either A2 or A9 peptide, or without any peptide (-) for 6 hours in the presence of a Syk inhibitor (Syk I) (10 pM), Piceatannol (300 μM), or DMSO as control. After culture, the cells were collected and the extracts were subjected to immunoblot analysis using an antibody against GATA-3 (top panel). The same membranes were then stripped and re-blotted with anti-β-actin antibody (lower panel). The results shown are representative of data obtained from three separate experiments. Densitometry of the results is displayed below the gel.