Skip to main content
. Author manuscript; available in PMC: 2009 Oct 5.
Published in final edited form as: J Am Soc Nephrol. 2004 May;15(5):1254–1260. doi: 10.1097/01.asn.0000125613.96927.38

Figure 5.

Figure 5

Immunoblotting of PDE5 protein abundance in cortical and IMCD cell homogenates from virgin, pregnant, and postpartum rat kidneys using a commercial antiserum to bovine lung PDE5. (Top) Representative immunoblot showing band intensity in IMCD cell but not cortical homogenates to be greater in samples from pregnant compared to virgin or postpartum rats; the product detected was of the expected size for rat PDE5 (98 kD). (Bottom) Scanning densitometry of immunoblots from 11 virgin, 11 pregnant, and 6 postpartum rats. PDE5 protein abundance was roughly 50% greater in IMCD cell homogenates from pregnant versus virgin or postpartum rat kidneys (*P < 0.05, one-way ANOVA), while no difference was detected in cortical homogenates. Data are means ± SEM.