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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Dev Biol. 2009 Jul 24;334(1):224–234. doi: 10.1016/j.ydbio.2009.07.025

Figure 3.

Figure 3

Morpholino knockdown of rpz suppresses the homozygous rapunzel phenotype. Heterozygous rapunzel adult zebrafish were intercrossed and the resulting embryos injected at the 1-2 cell stage with either a morpholino targeting the start site of rpz (MOrpz) (A), a morpholino targeting rpz2 (MOrpz2) (B) or a rpz 5 base pair mismatched control morpholino (MOrpz_5bm) (C). A. Injection of MOrpz suppresses the homozygous embryonic rapunzel phenotype. The rapunzel homozygous phenotype was not suppressed in embryos injected with MOrpz2 (B) or MOrpz_5bm (C). PCR, DdeI digestion and electrophoresis confirmed that the embryos in A, B, and C are all homozygous for the rapunzelc14 allele (not shown).