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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: J Immunol. 2009 Aug 26;183(6):3819–3830. doi: 10.4049/jimmunol.0901781

FIGURE 3.

FIGURE 3

Role of NF-κB activation on LPS-inducible interactions between the Igκ gene enhancers. Where indicated, 70Z/3 ± IκBΔN were treated with LPS for 20 h. A, The luciferase activity of a reporter plasmid whose expression was driven by κB sites was measured after transient transfection of the indicated cell lines. The level of activity is expressed as percent relative to the activity of a co-transfected pRL-CMV Renilla luciferase reporter gene. Standard deviations of three independent experiments are indicated. B, Real time RT-PCR assay of rearranged Vκ4 gene transcript levels. C, 3C assays of enhancer interactions. Standard deviations of three independent chromatin preparations are indicated.