Skip to main content
. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: J Immunol. 2009 Aug 26;183(6):3819–3830. doi: 10.4049/jimmunol.0901781

FIGURE 4.

FIGURE 4

Effect of cycloheximide and α-amanitin on LPS-inducible interactions between the Igκ gene enhancers. 70Z/3 cells were treated with cycloheximide and LPS for 5 h. Cycloheximide was added 30 min before LPS treatment. (A) Gel mobility super shift (S.S.) assays reveal that RelA is activated by LPS treatment in the presence or absence of cycloheximide. (B) 3C assays reveal that LPS-inducible enhancer interactions occur in the presence or absence of cycloheximide. Standard deviations of three independent chromatin preparations are indicated. (C) Real-time RT-PCR assay of rearranged Vκ4 gene transcript levels in 70Z/3 cells treated with α-amanitin as indicated. α-Amanitin was added to 70Z/3 cells 10 h before 5 h of LPS treatment. (D) 3C assays reveal that LPS-inducible enhancer interactions occur in the presence or absence of α-amanitin. Standard deviations of three independent chromatin preparations are indicated.